Immunopathological Mechanisms of HumanT Cell Lymphotropic
نویسندگان
چکیده
The immunopathology of human T cell lymphotropic virus type 1 (HTLV-I) uveitis was addressed by using T cell clones (TCC) established from the intraocular fluid of patients with HTLV-I uveitis. Proviral DNA of HTLV-I was identified in 55 out of 94 (59%) or 13 out of 36 (36%) TCC from the ocular fluid or the peripheral blood of these patients, respectively. Most of HTLV-I-infected TCC had a CD3+ CD4+ CD8phenotype. HTLV-I infection on TCC was confirmed by analysis of the viral mRNA, nucleotide sequence, virusassociated proteins, and virus particles. HTLV-I-infected TCC, but not HTLV-I negative TCC, constitutively produced high amounts of IL-6 (1,336±1,050 pg/ml) and TNFa (289±237 pg/ml) in the absence of any stimuli. HTLVI-infected TCC from the ocular lesion also constitutively produced high amounts of IL-la (12,699 pg/ml), IL-2 (61 pg/ml), IL-3 (428 pglml), IL-8 (1,268 pg/ml), IL-10 (28 pg/ ml), IFN-y (5,095 pg/ml), and GM-CSF (2,886 pg/ml). Hydrocortisone, a drug effective in vivo for the treatment of HTLV-I uveitis, severely depressed cytokine production in vitro in most cases. In summary, the results demonstrated direct evidence of HTLV-I infection of the eye and suggest that cytokines produced by HTLV-I-infected T cells are responsible for the intraocular inflammation in patients with HTLV-I uveitis. (J. Clin. Invest. 1995. 95:852-858.)
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